Measuring Gene Expression I

Learning Objectives

Learning Objectives:
Identify the main steps in a differential expression workflow
Learn about the example dataset
Organize a computational project
Retrieve data from public databases
Distinguish between types of parallel analysis
Run tasks in parallel with GNU Parallel
Run tasks in parallel with SLURM arrays
Perform initial QC
Align data against a reference genome
Perform alignment QC
Manipulate alignments in SAM/BAM format
Manipulate annotation files in GTF/GFF3 format
Quantify gene expression using reference alignments and a genome annotation